Thisdigoxigenin-labeledoligonucleotideproberecognizeshuman28SribosomalRNAwithintissuesections(1,2).ThisprobecanbeusedasacontrolwhenrunningspecificRNAtargetingprobes.ItshouldbeusedtoassessRNAintegrityinFFPEtissuesections.WeakorlightstaininginatestsampleindicatesthatspecificallytargetedmRNAmaybecompromised.
Volume | 400µl,8ml |
---|---|
IntendedUse | ASR |
References:
1.LeeD,XiongS,XiongWC.GeneralintroductiontoinsituhybridizationprotocolusingnonrADIoactivelylabeledprobestodetectmRNAsontissuesections.MethodsMolBiol.2013;1018:165-74.
2.PaillassonS,etal.Insituhybridizationinlivingcells:detectionofRNAmolecules.ExpCellRes.199725:231(1):226-33.
3.WilkinsonDG.InSituHybridization,APracticalApproach,OxfordUniversityPress(1992)ISBN0199633274.
4.CenterforDiseaseControlManual.Guide:SafetyManagement,NO.CDC-22,Atlanta,GA.April30,1976“DecontaminationofLaboratorySinkDrainstoRemoveAzideSalts.”
5.ClinicalandLaboratoryStandardsInstitute(CLSI).ProtectionofLaboratoryWorkersfromOccupationallyAcquiredInfections;ApprovedGuideline-FourthEditionCLSIdocumentM29-A4Wayne,PA2014.