TheRatDetectionKitforAnti-MouseCD31hasbeendevelopedfordetectionofaratanti-mouseCD31primaryantibodyonmousetissues.Thiskitiscomposedofblockers,digestionenzyme,primaryantibodydiluent,HRP-Polymer,DABchromogenandhematoxylin.Allreagentssuppliedwithkitaresuitableforbothmanualandautomatedtechniques.TheCD31primaryantibodyisavailableseparately.Biocare’sRat-on-MouseHRP-PolymerDetectionisspeciallydesignedforusingratmonoclonalantibodiesonmousetissues.Thekitutilizesabiotin-freehorserADIshperoxidase(HRP)polymertechnologythatprovidesasignificantincreaseinstainingsensitivity.Ratprimaryantibodiesareadvantageousonmousetissuesbecausemouseadsorbedanti-ratdetectionsystemsdisplayminimumcross-reactivitytoendogenousmouseIgG.TheRatHRP-PolymerDetectioniscomprisedoftworeagents:aRatProbethatbindstotheratprimaryantibody;andanHRP-Polymerthatconjugatestotheprobe.BiocarehasdevelopedRodentBlockMformousetissues.ItisaspeciallyformulatedblockingreagentthatreducesnonspecificbackgroundstainingandsimultaneouslyblocksforendogenousmouseIgG.RodentBlockMisappliedpriortotheprimaryantibodyfor30minutes.
Volume | 6.0ml |
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