Biocare’sPromARK™seriesintroducesRabbit-on-CanineHRP-PolymerDetection.Thisdetectionisspeciallydesignedforusingrabbitantibodiesoncanineandfelinetissuesandutilizesbiotin-freehorserADIshperoxidase(HRP)polymertechnology.Theadvantagesofthispolymertechnologyareincreasedsensitivity,reductionofIHCsteps(noAvidin/BiotinblockorLink/Probe),andminimalcross-reactivitytoendogenouscanineandfelineIgG.TheRabbit-on-Caninedetectioncanbeusedwithparaffin-embeddedtissuesandcanbeperformedmanuallyoronautomatedstainingplatforms.TheadditionofproprietaryblockerstothedetectionreagentpermitstheuseofanyofBiocareMedical’sretrievalsolutions(Reveal/Borg/Diva)orenzymeswiththeeliminationofendogenousIgGornon-specificbackgroundstaining.TemperaturedependentprotocolscanbeconvenientlyperformedusingBiocare’sDecloakingChamber.TheRabbit-on-CanineHRP-PolymermaybecombinedwiththeMouse-on-CanineHRP-PolymertocreateauniversalpolymerdetectionthatwilllabelbothmouseandrabbitantibodieswithHRP.RefertoTechnicalNote#4foradditionalinformation.TheRabbit-on-CanineHRP-PolymermaybecombinedwiththeMouse-on-CanineAP-PolymertocreateadoublestainpolymerdetectionthatwilllabeltherabbitantibodywithHRPandthemouseantibodywithAP.RefertoTechnicalNotes#5and#6foradditionalinformation.