Biocare’sPromARK™seriesintroducesRabbit-on-CanineAP-PolymerDetection.Thisdetectionisspeciallydesignedforusingrabbitantibodiesoncanineandfelinetissuesandutilizesbiotin-freealkalinephosphatase(AP)polymertechnology.Theadvantagesofthispolymertechnologyareincreasedsensitivity,reductionofIHCsteps(noAvidin/BiotinblockorLink/Probe),andminimalcross-reactivitytoendogenouscanineandfelineIgG.TheRabbit-on-Caninedetectioncanbeusedwithparaffin-embeddedtissuesandcanbeperformedmanuallyoronautomatedstainingplatforms.TheadditionofproprietaryblockerstothedetectionreagentpermitstheuseofanyofBiocareMedical’sretrievalsolutions(Reveal/Borg/Diva)orenzymeswiththeeliminationofendogenousIgGornon-specificbackgroundstaining.TemperaturedependentprotocolscanbeconvenientlyperformedusingBiocare’sDecloakingChamber.TheRabbit-on-CanineAP-PolymermaybecombinedwiththeMouse-on-CanineHRP-PolymertocreateadoublestainpolymerdetectionthatwilllabeltherabbitantibodywithAPandthemouseantibodywithHRP.RefertoTechnicalNotes#5and#6foradditionalinformation.