Whenamousemonoclonalantibodyisdesiredforimmunohistochemicaldetectiononmurinetissues,thebiotinylatedsecondaryantibodyusedfordetectionwillbindtotheprimaryantibodyandwillbindtoendogenousmouseIgGinthetissue.TroublesomebackgroundstainingmayoccurfromboundmouseIgG.
Themajorityofprimaryantibodiesonthemarketarenotbiotinylated.Itisveryexpensiveandtimeconsumingtobiotinylateanantibody.Usually,aminimum2-5mgofantibodyisnecessaryfortheprocedure,andthetotalamount(mg/ml)oftheantibodyisreducedthroughtheprocess.
Tocircumventtheproblem,BiocarehasdevelopedtheMMBiotinylationKit.Thekitprovidesallthereagentsnecessarytobiotinylateaprimaryantibody.
Thebasicconceptofthekitisverysimple.Priortotheapplicationofprimaryantibody,theantibodyismixedwithabiotinylationreagentfor30minutes.Aslittleas5to10μlofantibodycanbeused.Oncethecomplexisformed,itcanbeimmediatelyappliedtothetissuespecimen.Thetissueisthenincubatedwithstreptavidin-peroxidase,andthecolor-reactionisdevelopedbyadiaminobenzidine/hydrogenperoxidechromogen-substrate(DAB).
ThesystemhasbeenspeciallyformulatedtoeliminateendogenousmouseIgGbackgroundstaining.Eventhemostdifficulttissuessuchaslymphatic,lungandkidneytissuesarevirtuallybackgroundfree.
IntendedUse | RUO |
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Volume | 6ml |
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